How to Access Cryo-EM as an External User
Step-by-step guide for researchers at institutions without cryo-EM capabilities.
Step 1: Identify Nearby Facilities
Use the CryoEM Services to find facilities within your region that accept external users. Filter by state or use our city-based search (e.g., /facilities/near/boston) to find facilities near you. Look for the 'External Access' badge — facilities marked 'Internal Only' generally do not accept outside researchers.
Step 2: Understand Fee Structures
External user rates are typically 2-3x higher than internal rates. Most facilities require a service agreement or purchase order before granting access. Some offer a 'new user' or 'pilot project' rate for first-time users. Industry users pay the highest rates, often with a separate rate card.
Step 3: Apply for Access
Contact the facility manager (listed on each facility's directory page) to inquire about access. You'll typically need: an institutional purchase order or credit card authorization, a brief project description, proof of cryo-EM training (or willingness to be trained), and potentially a safety certification for your institution.
Step 4: Prepare Your Samples
Most facilities require you to arrive with purified, concentrated protein (typically 1-5 mg/mL in 20-50 µL). Some facilities offer sample preparation services (grid freezing with Vitrobot) while others expect you to prepare grids yourself. Confirm sample requirements before your session.
Alternative: Use a Private CRO
If academic facilities have long wait times or don't accept external users, private CROs offer end-to-end cryo-EM services: you ship your sample, they handle everything from grid preparation to 3D reconstruction. Use our Fast-Track Quote tool to get pricing from vetted CROs.
Frequently Asked Questions
Do I need training to use a cryo-EM facility?
Most facilities require some training, even for 'assisted' sessions. Training ranges from a half-day orientation to multi-day hands-on courses. Some facilities offer fully 'service' mode where their staff operate the microscope for you.
How long is the typical wait time?
Wait times vary from 2-12 weeks depending on the facility and instrument. Titan Krios time is most in-demand, with some top facilities booked 2-3 months in advance. Glacios and screening instruments typically have shorter queues.
Need Help Finding a Facility?
Browse our directory or get a fast-track quote from a private CRO.